The lab says fixation will lead to the dyes to leak within the cells. I feel the idea is that fixation may well problems cell membranes. I did look for a paper which describes fixation working with methanol or acetic acid, following staining which has a DCFDA by-product. DCFDA is https://2-fdckhcl71592.prublogger.com/28903320/5-simple-techniques-for-2-fdck-hcl